Ecotoxicology | OECD 211

GLP-Compliant Chronic Daphnia Toxicity Testing for Regulatory Environmental Risk Assessment

The OECD 211 Daphnia magna Reproduction Test is a key chronic ecotoxicity study used to evaluate the long-term effects of chemicals on aquatic invertebrates. The study determines whether prolonged exposure affects the survival and reproductive capacity of Daphnia magna, one of the most sensitive and internationally accepted test organisms in aquatic environmental risk assessment.

At LAUS, we perform GLP-compliant OECD 211 studies according to the latest OECD Test Guideline and support customers with reliable data for regulatory submissions worldwide.

Why is the OECD 211 Test Required?

The OECD 211 study provides information on chronic aquatic toxicity. It detects sublethal effects that may impair population sustainability.

The study is commonly required for:

·      REACH registrations

·      CLP and GHS

·      Biocidal Products Regulation (BPR)

·      Plant Protection Products

·      Environmental Risk Assessment (ERA) for Human Medicinal Products

·     Environmental classification and risk assessment of industrial chemicals  

The generated data are frequently used for PNEC derivation (Predicted No-Effect Concentration), environmental hazard assessment and regulatory decision-making.

OECD 211 Test Principle

In the OECD 211 Daphnia magna Reproduction Test, young female Daphnia magna are exposed to a range of test concentrations under controlled laboratory conditions for 21 days.

Throughout the study, the reproductive performance of exposed animals and those in untreated controls is monitored. Based on the findings a concentration-response relationship is determined.

Test Species

Daphnia magna

Regulatory Endpoints

·      NOEC (No Observed Effect Concentration)

·      LOEC (Lowest Observed Effect Concentration)

·     EC10 (10% Effect Concentration)

·    EC50 (Median Effect Concentration) on demand  

Specialised Ecotoxicity Testing for Complex Test Items

Reliable ecotoxicity testing begins with an appropriate exposure strategy. LAUS has extensive experience handling complex test materials, including poorly soluble substances, UVCBs, volatile compounds, surface-active substances, coloured products, difficult-to-disperse materials, complex formulations and multi-constituent substances.

Our study directors select the most suitable dosing and exposure approach for each project. Depending on the test item, this may include static or semi-static performance, Water Accommodated Fractions (WAF), solvent-assisted preparation or other scientifically justified techniques to achieve representative exposure conditions.

Study Scope According to OECD 211

LAUS has many years of experience performing GLP ecotoxicology studies for international regulatory submissions.

Our services include:

·      OECD 211 Daphnia magna Reproduction Test

·      Study design adapted to regulatory requirements

·      Static and semi-static exposure systems

·      Analytical concentration verification

·      GLP-compliant reporting

·     Scientific support throughout the study

As an independent GLP laboratory, we generate robust and reproducible data accepted by regulatory authorities worldwide.

Request a Quote

Looking for a reliable GLP laboratory for OECD 211 Daphnia magna Reproduction Tests?

Our experts are happy to discuss your project and provide a customized study proposal tailored to your regulatory requirements.