Mutagenicity and Genotoxicity
Mutagenicity and Genotoxicity
For the evaluation of genotoxic and/or mutagenic effects LAUS offers the following test methods:
| Guideline | Description |
|---|---|
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Ames Treat & Wash: modified procedure for amino acid-containing material |
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Ames MITI: for Japanese Ministry of International Trade and Industry |
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Ames MPF: miniaturized assay for extreme small amounts (screening) |
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OECD 473 |
In Vitro Mammalian Chromosome Aberration Test |
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OECD 487 |
In Vitro Mammalian Cell Micronucleus Test |
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OECD 490 |
In Vitro Mammalian Cell Gene Mutation Test: Mouse Lymphoma Test |
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OECD 476 |
Hypoxanthine-guanine phosphoribosyl transferase Test: HPRT Test |
The selection of a suitable test systems often depends on the test substance as well as the regulatory requirements. For nanomaterials, for example, exposure and proof of uptake into the cells are central components of the study. We will gladly develop a customized protocol with you to address specific properties of the test substance or to meet specific requirements.
Since a single test is not capable to detect all relevant genotoxic/mutagenic agents, the usual approach is to conduct a battery of tests of different focuses. Therefore, the tests are separated in three different categories:
- Testing of gene mutations on bacteria
- Testing of numerical and/or structural chromosome aberrations (aneugenic and clastogenic effects) on mammalian cells/human lymphocytes
- Testing of gene mutations on mammalian cells
In addition to human lymphocytes, the following cell lines are available:
- TK6 CRL8015 (human lymphoblast cell line isolated from spleen)
- L5178Y TK+/- clone (3.7.2C) (mouse lymphocytes)
- V79-4 (hamster lung fibroblasts)
The study you need is not listed? Please contact us. We’ll prepare you an individual offer for your needs.
